Acid-induced gelation of enzymatically cross-linked caseinate in different ionic milieus

Acid-induced gelation of enzymatically modified, preheated whey proteins.

Low-pH whey protein gels are formulated using a sequential protocol of heat treatment, enzyme incubation, and cold-set acidification. The heat-induced disulfide and enzyme-catalyzed epsilon-(gamma-glutamyl)lysine linkages, both at neutral pH, produce a polymerized protein solution. The molecular weights of these samples show an exponential increase with protein concentration. The additional enz...

Acid-Induced Aggregation and Gelation of Bovine Sodium Caseinate- Carboxymethylcellulose Mixtures

Enzymatically cross-linked hydrogels and their adhesive strength to biosurfaces.

OBJECTIVES To design an in-situ gelling hydrogel capable of solidifying rapidly under physiologic conditions into a hydrogel capable of adhering tissue surfaces together. DESIGN Multifunctional polymers containing covalently bound peptide substrates of transglutaminase were designed. EXPERIMENTAL VARIABLE Enzyme cross-linked hydrogels were compared with commercial fibrin tissue adhesive. ...

Magnetic removal of acid dyes from wastewater with ionic liquid linked-nanoparticles

The acid dyes are one of the most widely used commercial dyes used in the textile industries. This work investigates the ability of magnetite nanoparticles functionalized with imidazolium based ionic liquid as an efficient sorbent for removal of the Acid Blue 113 from wastewater. Fourier Transform Infrared Spectroscopy FT-IR, X-ray diffraction XRD and Transmission Electron Microscopy TEM were u...

Protein analysis by 31p NMR spectroscopy in ionic liquid: quantitative determination of enzymatically created cross-links.

Cross-linking of β-casein by Trichoderma reesei tyrosinase (TrTyr) and Streptoverticillium mobaraense transglutaminase (Tgase) was analyzed by (31)P nuclear magnetic resonance (NMR) spectroscopy in ionic liquid (IL). According to (31)P NMR, 91% of the tyrosine side chains were cross-linked by TrTyr at high dosages. When Tgase was used, no changes were observed because a different cross-linking ...